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Cloning & Synthetic Biology
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Epigenetics
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Product Categories
Restriction Endonucleases
PCR, qPCR & Amplification Technologies
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Sample Prep for NGS & Target Enrichment
Nucleic Acid Purification
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Genome Editing
Cellular Analysis
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Buffers
Strains
Discontinued
New Products
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Product Price List
Download a PDF containing pricing for our full
product list.
You’ll be Thrilled to Pieces
Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new
NEBNext® Ultra™ II FS DNA Library Prep Kit
with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability.
Learn more and request a sample!
Tools & Resources
Tools
NEBNext
®
Selector
NEBcloner
®
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®
Thermostable Ligase Reaction Temperature Calculator
NEBuilder
®
Assembly Tool
Enzyme Finder
Double Digest Finder
NEBcutter
®
REBASE
®
DNA Sequences and Maps Tool
Tm Calculator
PCR Fidelity Estimator
PCR Selector
PolBase
®
NEB Golden Gate Assembly Tool
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®
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Save time
and money by
placing an order
with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 8pm EST for overnight delivery.
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240 County Road
Ipswich, MA 01938-2723
978-927-5054
(Toll Free) 1-800-632-5227
Fax: 978-921-1350
Info@neb.com
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Contact Information
240 County Road
Ipswich, MA 01938-2723
978-927-5054
(Toll Free) 1-800-632-5227
Fax: 978-921-1350
Info@neb.com
Applications & Products
Applications
Cloning & Synthetic Biology
DNA Amplification, PCR and qPCR
Genome Editing
RNA Analysis
Sample Prep for NGS & Target Enrichment
Epigenetics
Protein Expression & Purification
Protein Analysis & Tools
Glycobiology & Proteomics
Cellular Analysis
Product Categories
Restriction Endonucleases
PCR, qPCR & Amplification Technologies
DNA Modifying Enzymes
Sample Prep for NGS & Target Enrichment
Nucleic Acid Purification
Markers & Ladders
RNA Reagents
DNA Assembly, Cloning and Mutagenesis Kits
Genome Editing
Cellular Analysis
Epigenetics
Protein Expression & Purification Technologies
Competent Cells
Protein Tools
Glycobiology
DNA Plasmids & Substrates
Buffers
Strains
Discontinued
New Products
Special Offers
Product Price List
Download a PDF containing pricing for our full
product list.
You’ll be Thrilled to Pieces
Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new
NEBNext® Ultra™ II FS DNA Library Prep Kit
with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability.
Learn more and request a sample!
Tools & Resources
Tools
NEBNext
®
Selector
NEBcloner
®
NEBioCalculator
®
Thermostable Ligase Reaction Temperature Calculator
NEBuilder
®
Assembly Tool
Enzyme Finder
Double Digest Finder
NEBcutter
®
REBASE
®
DNA Sequences and Maps Tool
Tm Calculator
PCR Fidelity Estimator
PCR Selector
PolBase
®
NEB Golden Gate Assembly Tool
NEBaseChanger
®
NEBnow Locator
Read Coverage Calculator
Competitor Cross Reference
Resources
FAQs
Protocols
Selection Charts
Troubleshooting Guides
Usage Guidelines/Tips
Interactive Tools
Video Library
Webinars
Application Notes
Order Catalog
Free Shipping
Save time
and money by
placing an order
with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 8pm EST for overnight delivery.
Support
Technical Support
US Customer & Order Support
International Ordering & Support
Customer Suggestions & Feedback
Find My Local US Sales Representative
New Lab/BioTech Discount
Catalog & Request Literature
NEBnow® Freezer Program
NEBsolutions® / OEM
Biotech Support
Student Services & Materials
Packaging & Shipping
Quality Assurance
Customer Suggestions & Feedback
Technical Support
Contact Information
240 County Road
Ipswich, MA 01938-2723
978-927-5054
(Toll Free) 1-800-632-5227
Fax: 978-921-1350
Info@neb.com
About
NEB Overview
News & Press Releases
Leadership
Research at NEB
Environmental Commitment
Quality at NEB
ISO Certification
Passion in Science Award
Business Development Opportunities
Careers
Contact Us
Contact Information
240 County Road
Ipswich, MA 01938-2723
978-927-5054
(Toll Free) 1-800-632-5227
Fax: 978-921-1350
Info@neb.com
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Demo Category
Are some ligations more difficult than others?
Whether DNA fragments have blunt ends or single-base overhangs, Greg explains why they can be difficult to ligate.
May-11-2017
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