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Quick Tips - How do I calculate how much DNA to add to a ligation reaction?
This video provides tips for calculating the ideal insert:vector molar ratio before performing a ligation reaction.
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Aug-30-2019
Here are some important things to keep in mind when preparing the NEBNext Ultra II FS DNA reaction buffer and enzyme mix for use.
Quick Tips - Preparing the NEBNext Ultra II FS DNA Reaction Buffer and Enzyme Mix
Aug-30-2019
Here are some important things to keep in mind when preparing the NEBNext Ultra II FS DNA reaction buffer and enzyme mix for use.
Quick Tips - Preparing the Ultra II FS DNA reaction buffer and enzyme mix
Apr-23-2019
Learn why it is important that the Bead Reconstitution Buffer be at room temperature for use.
Quick Tips - Bead Reconstitution Buffer for NEBNext Single Cell/Low Input RNA Library Prep
Apr-23-2019
Learn why vortexing the NEBNext Single Cell RT Buffer is so important.
Quick Tips - Vortexing the NEBNext Single Cell/Low Input RNA Library Prep Kit RT Buffer
Apr-23-2019
Learn how to make a master mix of the NEBNext Ultra II FS DNA reaction buffer and enzyme mix.
Quick Tips - Preparing NEBNext Ultra II FS DNA Reaction Buffer and Enzyme Mix Master Mix
Apr-23-2019
Learn how to make a master mix of the NEBNext Ultra II FS DNA reaction buffer and enzyme mix.
Quick Tips - Preparing NEBNext Ultra II FS DNA Reaction Buffer and Enzyme Mix Master Mix
Sep-24-2018
Learn how electrocompetent cells and chemically competent cells differ in the way uptake of DNA is achieved.
What are the differences between electrocompetent and chemically competent cells?
Jul-11-2018
This video explains when it's necessary to use a long incubation time for your ligation reaction.
Quick Tips - When is a long incubation time necessary for ligation?
Jul-11-2018
This video explains the best temperature at which to perform your ligation reaction.
Quick tips - What is the ideal incubation temperature for ligation?
Jul-11-2018
This video explains the source of a potential white precipitate in your T4 DNA ligase buffer.